Presented by: Abdoljalil Ghiadi
Turkmen horse represents the most glorious history in the field of horse breeding industry which results in presenting commercially available modern horses in the current time. Since Turkmen horse has a definitive role in preserving and development of horses used for different purposes e.g. endurance, jumping, dressage, racing, horse shows, pure and cross breeding et al, a global genotyping project will be in demand between the Turkmen horse breeding centers around the world. Moreover, annual congresses held among the participating centers will be of high importance to cooperate in various scientific point of views such as horse genotyping and echocardiography and scientific horse ecotourism. …The project of Iran’s Turkmen horse genotyping has been performed by Turkmen Horse Breeding & Consulting Co (THBC), where the participation of Ministry Of Agriculture Jahad (Animal Breeding Research Center and North Khorasan Organization Of Agriculture Jahad) should be acknowledged, among a total of 585 individuals using microsatellite DNA markers while 80 individuals are selected for mythochondrial DNA and Y chromosome analysis.
The following abstract (short thesis) shows the procedures of trials done for above mentioned genotyping project among 235 horses by the title of ‘Using total allelic relationship to analyze molecular marker genotypes of Iran’s Turkmen horses’:
Ardeshir Nejati-Javaremi 1, Ghodrat Rahimi 2, Klaus Olek 3, Abdoljalil Ghiadi 4:
Determined numbers of Turkmen horses were genotyped according to ISAG standards using 12 microsatellite paternity markers. Genomic DNA samples were isolated from whole blood with the Nucleospin Blood Quick Pure Kit. Multiplex PCR reactions were carried out in a total volume of 30 µl, containing dNTP-mix, MgCl2, Thermo-start DNA polymerase and genomic DNA. The following markers were used: HMS6; HTG4; AHT4; VHL20; HTG6; HTG7; HMS3; HMS6; in the 8-plex PCR and HTG10; HMS2; ASB2; AHT5 in the 4-plex PCR. For each PCR reaction one primer was 5ʹ-end labeled with commercially fluorescent labels TAMRA, FAM and HEX. The PCR fragments were denaturated, separated on a ABI 377 sequencer and analyzed with Genescan software. A total of 94 alleles were detected across the 13 microsatellite loci analyzed. The average homozygosity was 8.9±1.83, ranging between 6 for HTG7 and 12 for HTG10. The average homozygosity was 0.25±0.08, ranging from 0.16 to 0.4. total allelic relationship was used to analyze marker genotypes. Genetic distances of each individual with the sampled population was measured. Average inbreeding of all individuals was measured at 1.65%.
Web Resource: Turkmen Horse Breeding & Consulting Co.